Mia Park, Erika Garrido and Samya Clarke

Abstract:

Bacillus Subtilis is known for forming multicellular biofilms of varying morphological phenotypes, secreting a matrix composed of protein fibers and polysaccharides. Previously, we determined that the biofilm architecture changed in strains that had adapted to the laboratory. Here, we describe differences between colony architecture of B. subtilis grown on two different biofilm inducing media: LBGM, and MsGG. We also identified specific genes mutated in laboratory-adapted strains that influence biofilm morphology one or both media. Although we observed differences in morphology between media, further testing is required to fully characterize specific mechanisms.