Aretha Fraser and Jack Fox

Abstract:

Lead poisoning is a preventable U.S. health issue, often from lead-based paint, contaminated water, soil, or occupational exposure. Around 2.5% of young children are affected by lead poisoning, and over 170 million adults were exposed in childhood, leading to lasting developmental, cognitive, and chronic health problems. One proposed mechanism involves lead (Pb²⁺) disrupting neurotransmitter release by binding to synaptotagmin-1 (syt-1), a calcium-sensing protein essential for vesicle fusion with the cell membrane via SNARE proteins. This interference blocks synaptic communication. The study focuses on understanding this mechanism by examining Pb²⁺’s effects on syt-1 structure and function, with this summer’s work centered on expressing and purifying syt-1 in E. coli.